Dear mothur team,
I am trying to demultiplex the seq files of a MiSeq run, which have forward.fastq, reverse.fastq and one index.fastq.
example lines from the forward.fastq:
@MISEQ:192:000000000-A88BF:1:1101:14475:1417 1:N:0:CCCTCTTTTTTC
TCCTTCTTCTCCTCTCTTTCTCCTTCTTTCTTTTTTTTCCCTTTCTCTTCTTCTTTTTTTTCCTTCCTTTTTTCCCTTTTTCTTCTCCCCCTTTCCCTTTCCTTTTCTCCTTTTTTTCTTTCTTTCCTTTTCTTTCTTCTTCCTTCCTTTTTTCTCTTTTCCCTTCTTCTCTCTCCCTCCTTCCTCCTCTTTCTCCTTCCTCTTCCTTTCCTTCCCCTTCCTCTTCTTCTCTCCCTCTTTTTTCTCCCCCC
example lines from the reverse.fastq:
@MISEQ:192:000000000-A88BF:1:1101:16318:1652 1:N:0:CACGCCATAATG
TCCTTCTTCTCCTCTCTTTCTCCTTCTTTCTTTTTTTTCCCTTTCTCTTCTTCTTTTTTTTCCTTCTTTTTTTCCCTTTTTCTTCTCCCCCTTTTCCCTTCTTTTCGTCCTTGTTTCCTTTCTTTCGCTCTATTTCTTCTTCCTTCCTTTTTTCTCTTTTCCCTTCTTCGCTCTCCCGCCGCACTCCACTTTCTCCTGCCTCTTCCTCCCCTTCCCCTTCCTCTTCTTCTCTCCCTTTTTTTTCTCCCCCC
example lines from the index.fastq:
@MISEQ:192:000000000-A88BF:1:1101:14475:1417 1:N:0:CCCTCTTTTTTC
CCCTCTTTTTTC
+
AAAAAAAAAAAA
@MISEQ:192:000000000-A88BF:1:1101:15818:1635 1:N:0:CTCCCCTCCTCT
CTCCCCTCCTCT
However, I couldn’t figure out how I should demultiplex this kind of data. Would you please help me with this?
Thank you very much for your help.