All Unclassified Eukaryota data

Commands in mothur
1

When I used Silva 138.1 reference, start=1380, end=1510 and used in the alignment of my fasta sequence reads. I have got mostly unclassified Eukaryota

What I am missing here for proper classification of sequence?

2

I’m pretty confident that you have the wrong start and end coordinates. What region did you sequence and what are you sequencing? Have you seen this tutorial?

3

Hi,
Thank you for the reply.
Yes, customizing the region solved the problem.

Thank you.

4

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