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Mixed 16S/ITS kit but reads look overwhelmingly V3–V4 — how to verify and subset per region?
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1
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67
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October 16, 2025
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Rarefaction in mothursop
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1
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123
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October 14, 2025
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Appropriate length filtering strategy for 16S V4–V5 (515F–907R) amplicons: percentile vs fixed cutoffs?
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3
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199
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October 9, 2025
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Error during pcr.seqs step: Error in reading your fastafile, at position -1. Blank name
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5
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136
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October 1, 2025
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You did not provide a class
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9
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182
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September 30, 2025
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UD Index's and amplicon length
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5
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121
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September 22, 2025
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Sub.sample output use
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1
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99
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September 20, 2025
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Compare Samples With Sequences of Different Lengths?
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3
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119
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August 25, 2025
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AMOVA Tutorial Sample design File Missing Headers
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0
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44
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August 25, 2025
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Issue with bacterial classification using Mothur v1.48.3 and SILVA v138.2
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2
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81
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August 19, 2025
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Align.seq Process Killed
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2
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83
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August 19, 2025
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Pcr.seqs command error
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1
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46
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August 1, 2025
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Classify.seqs() marking most sequences as unclassified
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2
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96
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July 28, 2025
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Convert mothur taxonomy to NCBI tax ID
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1
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53
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July 15, 2025
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Pre.cluster 18S
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3
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112
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July 7, 2025
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Different Results When Using Different Number of Processors (make.contigs() w/t v.1.37.4 and v.1.48.3)
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1
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94
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June 25, 2025
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Silva.bacteria.fasta file
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3
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159
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June 25, 2025
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Error in reading your fastafile, at position -1. Blank name
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7
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639
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June 13, 2025
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Translate.seqs other code tables?
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1
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49
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June 13, 2025
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18S Sequences Too Short After Alignment with SILVA nr_v138_2 – Mothur v1.48.0
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6
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146
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June 10, 2025
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Inquiry on 16S rRNA Analysis Using Mothur and Data Trimming Techniques
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1
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73
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June 4, 2025
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Opti_mcc file confusion
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3
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110
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May 20, 2025
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Merge.outs cannot merge for taxlevel 5(family)
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6
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148
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May 15, 2025
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Why use Consensus sequences
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3
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128
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May 14, 2025
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New SILVA file name
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1
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57
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May 14, 2025
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Cluster and cluster.split cannot convert to integer
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6
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135
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May 13, 2025
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How does sub.sample function works
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5
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173
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May 10, 2025
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Chimera vsearch error
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10
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556
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May 9, 2025
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Make.contigs runs out of memory
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5
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156
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May 6, 2025
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Sequence file lost!
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5
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82
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April 21, 2025
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