I am a beginner, and I want to use Mothur for the first time! My goal is to give my dataset to mothur, (a datafile containing 10000 sequences) and find the clusters!
I have been searching the website for a tutorial on how to basically do the required steps, reading in the data, preprocess and calculate the distances, and finally fo the clusters! On the wiki page, I see that there is a section on OTU-based approaches (http://www.mothur.org/wiki/OTU-based_approaches) which has lots of commands, and cluster is one of them, but I need to know where to start!
I think I need to learn the basics stuff, such as how does mothur, how to read the files, and the steps to cluster sequences!
Any help is much appreciated!