Hi Mothur team,
I was hoping you could help. I was hoping to sequence the V4 region (primers 515F - 806R) of my bacterial communities. I have read how you recommend the Illumina MiSeq v2 2x250 kit.
However, if I were to use the Illumina adapters for sequencing would I have to use the v3 kit?
Have you any experience sequencing the V4 region with illumina adapters instead of your custom adapters?