Hi Mothur Community,
I am hoping to pool a 16s V4 amplicon library (Schloss/Kozich method) of ~300 samples with an ITS2 amplicon library (~20 samples). Does anyone know if this would be possible? The ITS2 amplicon library is constructed similar to 16s (using the same adapter, barcode, pad… etc). Can the V4 and ITS sequencing primers be spiked into their respective MiSeq cartridge wells (12, 13 and 14) together? Or will there be a concern for primer interactions.
ITSIndex (RC of Read.2) CAAAGATTCGATGARTCACGGCTGACTGACT