Hi Pat and all the Mothur community-
I am trying to use commands like chop.seqs or trim.seqs to remove just the 10 first nucleotides in my contig fasta file, but none of this seems to do exactly what I need. My sequences are not all having the same sizes, so using the option keep= first or keep=back won’t return all the sequences with only the first 10 nucleotides trimed.
Any suggestion, please?
Solved!!! I added NNNNNNNNNN following the forward primer before using the trim.seq command.
Best!
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