Which start and end point should I use to trim the reference to V3-V4 region? Is there a map table or something?
I’v tried to use pcr.seqs with V3-V4 primers but failed due to the gaps.
Or maybe I can remove all the gaps, trim it to V3-V4 region, and align it to something to get the gaps back?
May be you are looking for this…
Thanks, it’s helpful.