too many interactions

I’m running a PCA analysis using 2493 sequences and Mothur is processing it for five hours, with the follow mensage: “Too many iterations in tqli”. I’ve tried in two computers and the same mensage appeared. What should I do to solve this problem?

Thank you!

Can you post the command as you are running it?


To be exactly: mothur>pca(,label=0.03)

Thank you!

This message is displayed when mothur has tried over 3000 iterations to find the convergence with no success. If you send your shared file to, I can take a closer look.

Dear, thank you for your answer. We detected an error in the sequence cleaning. After solve this problem the analysis are running quite well. Thank you again.