Shared file numOtus column number wrong

mothur bugs
1

Hello,

I am having trouble with the count.groups function (or any function that uses the shared file). I get the following prompt:

mothur > count.groups(shared = ./lefse/lefse_genus_exp_control.shared)
[ERROR]: your shared file contains 526 OTU labels, but your numOtus column indicates 525. Cannot continue, please correct. This can be caused by editing your file incorrectly outside of mothur.
Q_16_16s contains 17652.
Q_17_16s contains 17556.
Q_18_16s contains 17627.
Q_1_16s contains 17651.
Q_25_16s contains 15990.
Q_26_16s contains 17659.
Q_27_16s contains 17630.
Q_28_16s contains 17595.
Q_29_16s contains 17602.
Q_2_16s contains 17672.
Q_30_16s contains 17530.
Q_3_16s contains 17664.
Q_49_16s contains 17731.
Q_4_16s contains 17685.
Q_50_16s contains 17702.
Q_51_16s contains 17725.
Q_52_16s contains 17743.
Q_53_16s contains 17721.
Q_54_16s contains 17725.
Q_55_16s contains 17649.
Q_56_16s contains 17608.
Q_57_16s contains 17637.
Q_58_16s contains 17700.
Q_59_16s contains 17684.
Q_5_16s contains 17635.
Q_60_16s contains 17706.
Q_61_16s contains 17717.
Q_62_16s contains 17699.
Q_63_16s contains 17731.
Q_64_16s contains 17736.
Q_65_16s contains 17394.
Q_66_16s contains 17733.
Q_67_16s contains 17615.
Q_68_16s contains 17690.
Q_69_16s contains 17645.
Q_6_16s contains 17545.
Q_70_16s contains 17636.
Q_71_16s contains 17669.
Q_72_16s contains 17665.
Q_75_16s contains 17673.
Q_76_16s contains 17662.
Q_77_16s contains 17728.
Q_81_16s contains 17692.
Q_82_16s contains 17661.
Q_83_16s contains 17679.
Q_84_16s contains 17701.
Q_85_16s contains 17710.
Q_86_16s contains 17713.
Q_87_16s contains 17717.
Q_88_16s contains 17707.
Q_89_16s contains 17646.

Size of smallest group: 15990.

Total seqs: 899243.

Output File Names: 
/home/ExtraDrive3/phdstudent2/CEAEQ_2024_16S/trimmed/final_quebec_16s/lefse/lefse_genus_exp_control.count.summary

I am sure I only have 525 labels. Any idea why mothur is saying I got 526 and not 525?
Is there any function that returns the OTUs detected by mothur?

Thanks!

Regards,
Elliston

2

Could you post the first 2 lines of the shared file?

3
label Group numOtus Pseudomonas Erythrobacter Methylophilus Bacteria.unclassified Methylobacterium Limnobacter Blastomonas Methylorubrum Cupriavidus Sphingopyxis Sphingomonas Cyanobacteriota.unclassified Flavobacterium Gammaproteobacteria.unclassified Methyloversatilis Hydrogenophaga Bradyrhizobiaceae.unclassified Nevskia Novosphingobium Pseudomonadaceae.unclassified Skermanella Microbacteriaceae.unclassified Hyphomicrobiaceae.unclassified Sphingobium Sediminibacterium Flectobacillus Comamonadaceae.unclassified Mycobacterium Alphaproteobacteria.unclassified Enterobacter Brevundimonas Bosea Afipia Legionella Methylophilaceae.unclassified Emticicia Oxalobacteraceae.unclassified Aquella Sphingomonadaceae.unclassified Algoriphagus Perlucidibaca Pedobacter Noviherbaspirillum Bowmanella Pseudoxanthomonas Sphingomonadales.unclassified Dyadobacter Stenotrophomonas Gemmatimonas Ferrovibrio Polycyclovorans Reyranella Rhizorhabdus Hyphomicrobium Peredibacter Hyphomicrobiales.unclassified Caryophanales.unclassified Paracoccaceae.unclassified Pseudomonadota.unclassified Comamonas Rhodopseudomonas Chryseotalea Aeromonas Rheinheimera Cellvibrio Betaproteobacteria.unclassified Chryseobacterium Arenimonas Luteimonas Herminiimonas Arcicella Acidovorax Erythrobacteraceae.unclassified Bacteroidota.unclassified Chitinophagaceae.unclassified Acinetobacter Aquabacterium Shinella Nubsella Panacagrimonas Myxococcales.unclassified Terrimicrobium Bdellovibrionaceae.unclassified Phenylobacterium Niveispirillum Stappia Roseomonas Aquicella Silanimonas Nevskiaceae.unclassified Spirosoma Thermoactinomycetaceae.unclassified Caulifigura Sphingorhabdus Achromobacter Opitutaceae.unclassified Runella Planctomycetia.unclassified Stenotrophobacter Orbaceae.unclassified Brevifollis Tsuneonella Geminicoccus Lacibacter Cyanophyceae.unclassified Zoogloeaceae.unclassified Rariglobus Pseudomethylobacillus Neochlamydia Cytophaga Exiguobacterium Vitreimonas Planctopirus Yersiniaceae.unclassified Bdellovibrio Pantoea Parachlamydia Urbifossiella Acetobacteraceae.unclassified Bradyrhizobium Pirellulales.unclassified Pseudobdellovibrio Rhodocyclales.unclassified Caulobacter Acidobacteriota.unclassified Rhodocyclaceae.unclassified Fluviicola Phyllobacteriaceae.unclassified Mesorhizobium Pseudohongiella Blastocatellaceae.unclassified Terricaulis Sphingosinicella Botrimarina Turicibacter Lignipirellula Flavobacteriaceae.unclassified Prosthecobacter Chlamydiales.unclassified Pirellulaceae.unclassified Pirellula Acetobacteroides Pseudonocardia Variovorax Azospira Planctomycetaceae.unclassified Singulisphaera Flavihumibacter Daejeonella Parachlamydiaceae.unclassified Oleiharenicola Sphingobacterium Burkholderiales.unclassified Blastocatella Yersinia Massilia Elizabethkingia Arenibacter Gp6 Devosia Gloeobacterales.unclassified Methylophaga Klenkia Asprobacter Crocinitomicaceae.unclassified Myxococcaceae.unclassified Enterococcus Gemmataceae.unclassified Luteolibacter Terrimonas Bacillota.unclassified Hyphomonas Thalassospira Flavobacteriales.unclassified Cyanobium Chroococcales.unclassified Panacibacter Corynebacterium Isosphaeraceae.unclassified Dissulfurispira Pigmentiphaga Cloacibacterium Cavicella Sphingoaurantiacus Fulvivirgaceae.unclassified Microbacterium Xanthomonadales.unclassified Saccharibacteria.genera.incertae.sedis Rubellimicrobium Rhodospirillales.unclassified Rhizobacter Subdivision3.genera.incertae.sedis Sandaracinus Tahibacter Turneriella Simplicispira Bryobacter Salinisphaera Clostridium.sensu stricto Ohtaekwangia Rhodoplanes Deltaproteobacteria.unclassified Chromatiales.unclassified Desulfobacteraceae.unclassified Siccirubricoccus Paraclostridium Rickettsiaceae.unclassified Photobacterium Rhodanobacteraceae.unclassified Caulobacterales.unclassified Xanthomonadaceae.unclassified Gaiella Providencia Cystobacter Ramlibacter Bacteriovoracales.unclassified Enterobacterales.unclassified Lacipirellulaceae.unclassified Hymenobacter Micrococcaceae.unclassified Thalassolituus Romeriopsis Qipengyuania Halomonas Thermaceae.unclassified Lacipirellula Haliscomenobacter Staphylococcus Caulobacteraceae.unclassified Phaeobacter Truepera Kocuria Rudanella Gemmatales.unclassified Lawsonella Aggregatilinea Raineya Micropepsaceae.unclassified Mucilaginibacter Clostridiaceae.1.unclassified Chryseolinea Morganella Porticoccus Asticcacaulis Methylobacteriaceae.unclassified Streptococcus Marinobacter Sandarakinorhabdus Pseudofulvimonas Flavitalea Methylocystis Nocardioidaceae.unclassified Agromyces Geminicoccaceae.unclassified Nitrospira Foliisarcina Phnomibacter Sneathiellaceae.unclassified Belnapia Simkania Paenibacillus Hydrotalea Acidibacter Methylotenera Bacteroidales.unclassified Occidentia Proteus Methylococcaceae.unclassified Rhodobacterales.unclassified Romboutsia Sporosarcina Cytophagales.unclassified Paeniclostridium Rubrivirga Rickettsia Gp7 Longimicrobium Marmoricola Actinotalea Gemmatimonadaceae.unclassified Aurantimonadaceae.unclassified Fimbriiglobus Roseibium Schlesneria Thermoleophilia.unclassified Erysipelotrichaceae.unclassified Megasphaera Anaerocella Sphingobacteriaceae.unclassified Larkinella Syntrophobacteraceae.unclassified Geobacteraceae.unclassified Thiobacillus Asaia Rhodococcus Nostocales.unclassified Stappiaceae.unclassified Spartobacteria.genera.incertae.sedis Lysobacter Hoeflea Anaerolineae.unclassified Enterobacteriaceae.unclassified Rhizobiaceae.unclassified Steroidobacteraceae.unclassified Magnetospirillum Chloroflexota.unclassified Minwuia Dermacoccus Georhizobium Hydrocarboniphaga Chiayiivirga Roseobacteraceae.unclassified Gemella Curvibacter Syntrophaceae.unclassified Moraxella Paludibacter Roseateles Aquisphaera Terrihabitans Beijerinckia Dongia Actinobacteria.unclassified Marinicaulis Parvularculaceae.unclassified Gp3 Zavarzinella Flavilitoribacter Dechloromonas Miltoncostaea Streptomyces Nocardioides Actinomycetota.unclassified Paludihabitans Solimonas Mycobacteriales.unclassified Fibrella Acidimicrobiales.unclassified Parasegetibacter Solirubrobacterales.unclassified Halieaceae.unclassified Meiothermus Anaerolineaceae.unclassified Litorilinea Kordiimonas Wohlfahrtiimonas Rothia Deinococcus Abditibacterium Lacihabitans Thermoguttaceae.unclassified Tautonia Azospirillum Hafnia Desmonostoc Sporichthya Pectobacteriaceae.unclassified Rurimicrobium Pseudarthrobacter Pedomicrobium Azospirillaceae.unclassified Ignavibacterium Thermogemmata Demequina Coxiella Desulfosporosinus Gp16 Vagococcus Arthrobacter Aphanizomenonaceae.unclassified Aureliella Bacillaceae.unclassified Aequorivita Sneathiella Aridibacter Oculatellaceae.unclassified Anaerocolumna Planococcus Verrucomicrobiota.unclassified Brevibacterium Peptoniphilus Alkanindiges Crocinitomix Geothrix Leptolyngbyaceae.unclassified Anaerococcus Lachnospiraceae.unclassified Altericista Verrucomicrobiaceae.unclassified Hyphobacterium Polyangium Polyangiaceae.unclassified Saprospirales.unclassified Thermoflavimicrobium Immundisolibacter Aminobacter Gracilibacter Buchnera Sphaerobacteraceae.unclassified Gp17 Eubacteriales.unclassified Pleurocapsales.unclassified Campylobacter Blastococcus Sedimentibacter Thauera Bacillus Phreatobacter Paenibacillaceae.unclassified Desnuesiella Spiroplasma Rectinema Azohydromonas Parageobacillus Terrisporobacter Aquihabitans Anaerolinea Empedobacter Geodermatophilus Thermomicrobia.unclassified Duganella Arcticibacter Holophagaceae.unclassified Jeotgalibacillus Lacrimispora Lactococcus Brevibacillus Coxiellaceae.unclassified Desulfuromonadales.unclassified Acetobacterium Fundidesulfovibrio Dietzia Owenweeksia Desulfomonile Mariniradius Myroides Mollicutes.unclassified Segatella Planifilum Humidesulfovibrio Croceifilum Intrasporangium Gp22 Niallia Microcystaceae.unclassified Anagnostidinema Gemmobacter Gp10 Actinomycetaceae.unclassified Polynucleobacter Phycisphaeraceae.unclassified Syntrophorhabdus Arsenicitalea Gemmata Lysinibacillus Finegoldia Rahnella Dokdonella Nocardia Tepidisphaera Stagnimonas Spirochaetales.unclassified Pseudorhodoferax Muriicola Chitinophaga Paenarthrobacter Micrococcales.unclassified Sporomusaceae.unclassified Arcobacter Planctomycetota.unclassified Solibacillus Pelomicrobium Intrasporangiaceae.unclassified Burkholderiaceae.unclassified Fulvivirga Zymobacter Ornithinimicrobium Beijerinckiaceae.unclassified Taibaiella Cellvibrionaceae.unclassified Sulfuricella Enterovirga Pseudolabrys Micrococcus Lentimicrobium Methylomonas Carnobacterium Hydrogenispora Aeromicrobium Nitrosomonadales.unclassified Chromobacterium Brucella Aestuariivirga Kineosporiaceae.unclassified Caldilineaceae.unclassified Subdivision3.incertae.sedis.unclassified Dysgonomonadaceae.unclassified Ferruginibacter Pelagibacterium Legionellales.unclassified Alteraurantiacibacter Kroppenstedtia Anaeromyxobacter Oscillospiraceae.unclassified Neisseria Nitrosomonas Thermaerobacter Rickettsiella Macellibacteroides Haliea Luteitalea Pseudonocardiaceae.unclassified Metalysinibacillus Aggregicoccus Desulforamulus Casimicrobium Piscinibacter Selenomonadales.unclassified Silvanigrellaceae.unclassified Propionivibrio Hoylesella Latescibacteria.genera.incertae.sedis Anaerovorax Chishuiella Rare
ASV Q_16_16s 525 465 1 0 35 0 13 74 392 3536 0 597 0 208 0 0 520 0 0 5524 16 0 45 0 372 538 0 672 0 7 0 512 82 32 100 1 0 3 0 8 0 0 1055 0 0 11 218 1010 178 3 0 0 1 2 0 224 0 0 0 28 0 33 0 0 0 0 0 58 0 1 422 0 0 14 230 0 6 0 0 98 0 0 0 0 0 0 0 25 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3 20 0 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 38 0 0 0 0 0 0 0 62 37 0 0 2 62 0 0 27 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 17 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
4
1 Pseudomonas
2 Erythrobacter
3 Methylophilus
4 Bacteria.unclassified
5 Methylobacterium
6 Limnobacter
7 Blastomonas
8 Methylorubrum
9 Cupriavidus
10 Sphingopyxis
11 Sphingomonas
12 Cyanobacteriota.unclassified
13 Flavobacterium
14 Gammaproteobacteria.unclassified
15 Methyloversatilis
16 Hydrogenophaga
17 Bradyrhizobiaceae.unclassified
18 Nevskia
19 Novosphingobium
20 Pseudomonadaceae.unclassified
21 Skermanella
22 Microbacteriaceae.unclassified
23 Hyphomicrobiaceae.unclassified
24 Sphingobium
25 Sediminibacterium
26 Flectobacillus
27 Comamonadaceae.unclassified
28 Mycobacterium
29 Alphaproteobacteria.unclassified
30 Enterobacter
31 Brevundimonas
32 Bosea
33 Afipia
34 Legionella
35 Methylophilaceae.unclassified
36 Emticicia
37 Oxalobacteraceae.unclassified
38 Aquella
39 Sphingomonadaceae.unclassified
40 Algoriphagus
41 Perlucidibaca
42 Pedobacter
43 Noviherbaspirillum
44 Bowmanella
45 Pseudoxanthomonas
46 Sphingomonadales.unclassified
47 Dyadobacter
48 Stenotrophomonas
49 Gemmatimonas
50 Ferrovibrio
51 Polycyclovorans
52 Reyranella
53 Rhizorhabdus
54 Hyphomicrobium
55 Peredibacter
56 Hyphomicrobiales.unclassified
57 Caryophanales.unclassified
58 Paracoccaceae.unclassified
59 Pseudomonadota.unclassified
60 Comamonas
61 Rhodopseudomonas
62 Chryseotalea
63 Aeromonas
64 Rheinheimera
65 Cellvibrio
66 Betaproteobacteria.unclassified
67 Chryseobacterium
68 Arenimonas
69 Luteimonas
70 Herminiimonas
71 Arcicella
72 Acidovorax
73 Erythrobacteraceae.unclassified
74 Bacteroidota.unclassified
75 Chitinophagaceae.unclassified
76 Acinetobacter
77 Aquabacterium
78 Shinella
79 Nubsella
80 Panacagrimonas
81 Myxococcales.unclassified
82 Terrimicrobium
83 Bdellovibrionaceae.unclassified
84 Phenylobacterium
85 Niveispirillum
86 Stappia
87 Roseomonas
88 Aquicella
89 Silanimonas
90 Nevskiaceae.unclassified
91 Spirosoma
92 Thermoactinomycetaceae.unclassified
93 Caulifigura
94 Sphingorhabdus
95 Achromobacter
96 Opitutaceae.unclassified
97 Runella
98 Planctomycetia.unclassified
99 Stenotrophobacter
100 Orbaceae.unclassified
101 Brevifollis
102 Tsuneonella
103 Geminicoccus
104 Lacibacter
105 Cyanophyceae.unclassified
106 Zoogloeaceae.unclassified
107 Rariglobus
108 Pseudomethylobacillus
109 Neochlamydia
110 Cytophaga
111 Exiguobacterium
112 Vitreimonas
113 Planctopirus
114 Yersiniaceae.unclassified
115 Bdellovibrio
116 Pantoea
117 Parachlamydia
118 Urbifossiella
119 Acetobacteraceae.unclassified
120 Bradyrhizobium
121 Pirellulales.unclassified
122 Pseudobdellovibrio
123 Rhodocyclales.unclassified
124 Caulobacter
125 Acidobacteriota.unclassified
126 Rhodocyclaceae.unclassified
127 Fluviicola
128 Phyllobacteriaceae.unclassified
129 Mesorhizobium
130 Pseudohongiella
131 Blastocatellaceae.unclassified
132 Terricaulis
133 Sphingosinicella
134 Botrimarina
135 Turicibacter
136 Lignipirellula
137 Flavobacteriaceae.unclassified
138 Prosthecobacter
139 Chlamydiales.unclassified
140 Pirellulaceae.unclassified
141 Pirellula
142 Acetobacteroides
143 Pseudonocardia
144 Variovorax
145 Azospira
146 Planctomycetaceae.unclassified
147 Singulisphaera
148 Flavihumibacter
149 Daejeonella
150 Parachlamydiaceae.unclassified
151 Oleiharenicola
152 Sphingobacterium
153 Burkholderiales.unclassified
154 Blastocatella
155 Yersinia
156 Massilia
157 Elizabethkingia
158 Arenibacter
159 Gp6
160 Devosia
161 Gloeobacterales.unclassified
162 Methylophaga
163 Klenkia
164 Asprobacter
165 Crocinitomicaceae.unclassified
166 Myxococcaceae.unclassified
167 Enterococcus
168 Gemmataceae.unclassified
169 Luteolibacter
170 Terrimonas
171 Bacillota.unclassified
172 Hyphomonas
173 Thalassospira
174 Flavobacteriales.unclassified
175 Cyanobium
176 Chroococcales.unclassified
177 Panacibacter
178 Corynebacterium
179 Isosphaeraceae.unclassified
180 Dissulfurispira
181 Pigmentiphaga
182 Cloacibacterium
183 Cavicella
184 Sphingoaurantiacus
185 Fulvivirgaceae.unclassified
186 Microbacterium
187 Xanthomonadales.unclassified
188 Saccharibacteria.genera.incertae.sedis
189 Rubellimicrobium
190 Rhodospirillales.unclassified
191 Rhizobacter
192 Subdivision3.genera.incertae.sedis
193 Sandaracinus
194 Tahibacter
195 Turneriella
196 Simplicispira
197 Bryobacter
198 Salinisphaera
199 Clostridium.sensu
200 stricto
201 Ohtaekwangia
202 Rhodoplanes
203 Deltaproteobacteria.unclassified
204 Chromatiales.unclassified
205 Desulfobacteraceae.unclassified
206 Siccirubricoccus
207 Paraclostridium
208 Rickettsiaceae.unclassified
209 Photobacterium
210 Rhodanobacteraceae.unclassified
211 Caulobacterales.unclassified
212 Xanthomonadaceae.unclassified
213 Gaiella
214 Providencia
215 Cystobacter
216 Ramlibacter
217 Bacteriovoracales.unclassified
218 Enterobacterales.unclassified
219 Lacipirellulaceae.unclassified
220 Hymenobacter
221 Micrococcaceae.unclassified
222 Thalassolituus
223 Romeriopsis
224 Qipengyuania
225 Halomonas
226 Thermaceae.unclassified
227 Lacipirellula
228 Haliscomenobacter
229 Staphylococcus
230 Caulobacteraceae.unclassified
231 Phaeobacter
232 Truepera
233 Kocuria
234 Rudanella
235 Gemmatales.unclassified
236 Lawsonella
237 Aggregatilinea
238 Raineya
239 Micropepsaceae.unclassified
240 Mucilaginibacter
241 Clostridiaceae.1.unclassified
242 Chryseolinea
243 Morganella
244 Porticoccus
245 Asticcacaulis
246 Methylobacteriaceae.unclassified
247 Streptococcus
248 Marinobacter
249 Sandarakinorhabdus
250 Pseudofulvimonas
251 Flavitalea
252 Methylocystis
253 Nocardioidaceae.unclassified
254 Agromyces
255 Geminicoccaceae.unclassified
256 Nitrospira
257 Foliisarcina
258 Phnomibacter
259 Sneathiellaceae.unclassified
260 Belnapia
261 Simkania
262 Paenibacillus
263 Hydrotalea
264 Acidibacter
265 Methylotenera
266 Bacteroidales.unclassified
267 Occidentia
268 Proteus
269 Methylococcaceae.unclassified
270 Rhodobacterales.unclassified
271 Romboutsia
272 Sporosarcina
273 Cytophagales.unclassified
274 Paeniclostridium
275 Rubrivirga
276 Rickettsia
277 Gp7
278 Longimicrobium
279 Marmoricola
280 Actinotalea
281 Gemmatimonadaceae.unclassified
282 Aurantimonadaceae.unclassified
283 Fimbriiglobus
284 Roseibium
285 Schlesneria
286 Thermoleophilia.unclassified
287 Erysipelotrichaceae.unclassified
288 Megasphaera
289 Anaerocella
290 Sphingobacteriaceae.unclassified
291 Larkinella
292 Syntrophobacteraceae.unclassified
293 Geobacteraceae.unclassified
294 Thiobacillus
295 Asaia
296 Rhodococcus
297 Nostocales.unclassified
298 Stappiaceae.unclassified
299 Spartobacteria.genera.incertae.sedis
300 Lysobacter
301 Hoeflea
302 Anaerolineae.unclassified
303 Enterobacteriaceae.unclassified
304 Rhizobiaceae.unclassified
305 Steroidobacteraceae.unclassified
306 Magnetospirillum
307 Chloroflexota.unclassified
308 Minwuia
309 Dermacoccus
310 Georhizobium
311 Hydrocarboniphaga
312 Chiayiivirga
313 Roseobacteraceae.unclassified
314 Gemella
315 Curvibacter
316 Syntrophaceae.unclassified
317 Moraxella
318 Paludibacter
319 Roseateles
320 Aquisphaera
321 Terrihabitans
322 Beijerinckia
323 Dongia
324 Actinobacteria.unclassified
325 Marinicaulis
326 Parvularculaceae.unclassified
327 Gp3
328 Zavarzinella
329 Flavilitoribacter
330 Dechloromonas
331 Miltoncostaea
332 Streptomyces
333 Nocardioides
334 Actinomycetota.unclassified
335 Paludihabitans
336 Solimonas
337 Mycobacteriales.unclassified
338 Fibrella
339 Acidimicrobiales.unclassified
340 Parasegetibacter
341 Solirubrobacterales.unclassified
342 Halieaceae.unclassified
343 Meiothermus
344 Anaerolineaceae.unclassified
345 Litorilinea
346 Kordiimonas
347 Wohlfahrtiimonas
348 Rothia
349 Deinococcus
350 Abditibacterium
351 Lacihabitans
352 Thermoguttaceae.unclassified
353 Tautonia
354 Azospirillum
355 Hafnia
356 Desmonostoc
357 Sporichthya
358 Pectobacteriaceae.unclassified
359 Rurimicrobium
360 Pseudarthrobacter
361 Pedomicrobium
362 Azospirillaceae.unclassified
363 Ignavibacterium
364 Thermogemmata
365 Demequina
366 Coxiella
367 Desulfosporosinus
368 Gp16
369 Vagococcus
370 Arthrobacter
371 Aphanizomenonaceae.unclassified
372 Aureliella
373 Bacillaceae.unclassified
374 Aequorivita
375 Sneathiella
376 Aridibacter
377 Oculatellaceae.unclassified
378 Anaerocolumna
379 Planococcus
380 Verrucomicrobiota.unclassified
381 Brevibacterium
382 Peptoniphilus
383 Alkanindiges
384 Crocinitomix
385 Geothrix
386 Leptolyngbyaceae.unclassified
387 Anaerococcus
388 Lachnospiraceae.unclassified
389 Altericista
390 Verrucomicrobiaceae.unclassified
391 Hyphobacterium
392 Polyangium
393 Polyangiaceae.unclassified
394 Saprospirales.unclassified
395 Thermoflavimicrobium
396 Immundisolibacter
397 Aminobacter
398 Gracilibacter
399 Buchnera
400 Sphaerobacteraceae.unclassified
401 Gp17
402 Eubacteriales.unclassified
403 Pleurocapsales.unclassified
404 Campylobacter
405 Blastococcus
406 Sedimentibacter
407 Thauera
408 Bacillus
409 Phreatobacter
410 Paenibacillaceae.unclassified
411 Desnuesiella
412 Spiroplasma
413 Rectinema
414 Azohydromonas
415 Parageobacillus
416 Terrisporobacter
417 Aquihabitans
418 Anaerolinea
419 Empedobacter
420 Geodermatophilus
421 Thermomicrobia.unclassified
422 Duganella
423 Arcticibacter
424 Holophagaceae.unclassified
425 Jeotgalibacillus
426 Lacrimispora
427 Lactococcus
428 Brevibacillus
429 Coxiellaceae.unclassified
430 Desulfuromonadales.unclassified
431 Acetobacterium
432 Fundidesulfovibrio
433 Dietzia
434 Owenweeksia
435 Desulfomonile
436 Mariniradius
437 Myroides
438 Mollicutes.unclassified
439 Segatella
440 Planifilum
441 Humidesulfovibrio
442 Croceifilum
443 Intrasporangium
444 Gp22
445 Niallia
446 Microcystaceae.unclassified
447 Anagnostidinema
448 Gemmobacter
449 Gp10
450 Actinomycetaceae.unclassified
451 Polynucleobacter
452 Phycisphaeraceae.unclassified
453 Syntrophorhabdus
454 Arsenicitalea
455 Gemmata
456 Lysinibacillus
457 Finegoldia
458 Rahnella
459 Dokdonella
460 Nocardia
461 Tepidisphaera
462 Stagnimonas
463 Spirochaetales.unclassified
464 Pseudorhodoferax
465 Muriicola
466 Chitinophaga
467 Paenarthrobacter
468 Micrococcales.unclassified
469 Sporomusaceae.unclassified
470 Arcobacter
471 Planctomycetota.unclassified
472 Solibacillus
473 Pelomicrobium
474 Intrasporangiaceae.unclassified
475 Burkholderiaceae.unclassified
476 Fulvivirga
477 Zymobacter
478 Ornithinimicrobium
479 Beijerinckiaceae.unclassified
480 Taibaiella
481 Cellvibrionaceae.unclassified
482 Sulfuricella
483 Enterovirga
484 Pseudolabrys
485 Micrococcus
486 Lentimicrobium
487 Methylomonas
488 Carnobacterium
489 Hydrogenispora
490 Aeromicrobium
491 Nitrosomonadales.unclassified
492 Chromobacterium
493 Brucella
494 Aestuariivirga
495 Kineosporiaceae.unclassified
496 Caldilineaceae.unclassified
497 Subdivision3.incertae.sedis.unclassified
498 Dysgonomonadaceae.unclassified
499 Ferruginibacter
500 Pelagibacterium
501 Legionellales.unclassified
502 Alteraurantiacibacter
503 Kroppenstedtia
504 Anaeromyxobacter
505 Oscillospiraceae.unclassified
506 Neisseria
507 Nitrosomonas
508 Thermaerobacter
509 Rickettsiella
510 Macellibacteroides
511 Haliea
512 Luteitalea
513 Pseudonocardiaceae.unclassified
514 Metalysinibacillus
515 Aggregicoccus
516 Desulforamulus
517 Casimicrobium
518 Piscinibacter
519 Selenomonadales.unclassified
520 Silvanigrellaceae.unclassified
521 Propionivibrio
522 Hoylesella
523 Latescibacteria.genera.incertae.sedis
524 Anaerovorax
525 Chishuiella
526 Rare

You have 526 OTU labels. What version of mothur are you running? It looks like you ran the split.abund command with an older version of mothur. You can upgrade to our latest version, available here.

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