When we get our sequences pyrosequenced using 454 we have them do it off the 28F primer. Are our sequences then running 5’-3’ or 3’-5’?
5->3 = so you do not need to flip them
Thank you!
When we get our sequences pyrosequenced using 454 we have them do it off the 28F primer. Are our sequences then running 5’-3’ or 3’-5’?
5->3 = so you do not need to flip them
Thank you!