When I run mothur > make.contigs(ffasta=GORGE_R1.paired.trim.fasta, rfasta=GORGE_R2.paired.trim.fasta)
I have the next Error:
Using 1 processors.
Reading fastq data…
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142048
[WARNING]: did not find paired read for M02897_67_000000000-ABF9G_1_1101_15293_2 0360, ignoring.
[WARNING]: did not find paired read for M02897_67_000000000-ABF9G_1_1101_2907_80 36, ignoring.
[WARNING]: did not find paired read for M02897_67_000000000-ABF9G_1_1102_14610_1 1709, ignoring.
[WARNING]: did not find paired read for M02897_67_000000000-ABF9G_1_1102_14619_1 1732, ignoring.
[WARNING]: did not find paired read for M02897_67_000000000-ABF9G_1_1102_16773_4 946, ignoring.
[…]
Processing GORGE_R1.paired.trim.0ffastatemp (file 1 of 1) <
Making contigs…
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[…]
And after, at the end of running screen.seqs (mothur > screen.seqs(fasta=GORGE_R1.paired.trim.trim.contigs.fasta, summary=GORGE_R1.paired.trim.trim.contigs.summary, maxlength=600, maxambig=0, processors=16) I have the next error:
[ERROR]: found 142048 sequences in your fasta file, and 108626 sequences in your summary file, quitting.
Where is the problem?
I cannot do the next step with the rest of my samples if I haven’t this sample