Hi there!!!
after alignment I’ve got this result:
Using 8 processors.
Start End NBases Ambigs Polymer NumSeqs
Minimum: 0 0 0 0 1 1
2.5%-tile: 2 17017 403 0 4 10266
25%-tile: 2 17018 404 0 4 102657
Median: 2 17018 413 0 5 205314
75%-tile: 2 17018 424 0 5 307971
97.5%-tile: 4 17018 429 4 7 400362
Maximum: 17063 17065 459 104 220 410627
Mean: 94 16931 410 0 4
# of unique seqs: 218440
total # of seqs: 410627
It took 34 secs to summarize 410627 sequences.
Is the following screen.seqs command enough to discard ‘‘bad’’ sequences? Or, should I add maxlength and/or minlength option with this?
screen.seqs(fasta=current, count=current, maxambig=0, maxhomop=8, start=2, end=17018)
Thanks and Regards,
DC7