Running Mothur on HPC

I am new to the forum. Has anyone used HPC to analyze 16S RNA amplicon seq. with Mothur? If so, I would appreciate it if you could walk me through the steps. I used Mothur in the past on HPC using FileZilla and Putty; can I use it without FileZilla? What files or folders I need to run Mothur on HPC besides my sequencing data? Thanks.


Use MobaXterm.

This provide you ssh and sftp in one app. And it’s free for personal.


I’m not sure what you’re trying to do exactly, but I do have some general materials on running mothur on an HPC or Amazon. You might find these useful…

Let me know if you have any more specific questions…


Thank you, Pat. I have some 16S rRNA amplicon sequencing data that i would like to run on HPC. I wanted to know the steps and what other things I might need besides my sequenced data. Hopefully the links you sent me will help. Thank you so much.

Kind regards!

Jean Rene

If you or have a collaborator/PI working in a Canadian university, you can use also Compute Canada. For free.