Hi,
I am having exactly the same problem. In my case, barcodes, indexes were removed by the sequencing facilities and I used degenerate primers, so I used all the possibles combinations, is that correct?. My oligo.files looks like this:
primer AAACTTAAAGGAATTGGCGG ACGGGCGGTGTGTGC
primer AAACTTAAAGGAATTGGCGG ACGGGCGGTGTGTAC
primer AAACTTAAAGGAATTGACGG ACGGGCGGTGTGTGC
primer AAACTTAAAGGAATTGACGG ACGGGCGGTGTGTAC
primer AAACTTAAATGAATTGGCGG ACGGGCGGTGTGTGC
primer AAACTTAAATGAATTGGCGG ACGGGCGGTGTGTAC
primer AAACTTAAATGAATTGACGG ACGGGCGGTGTGTGC
primer AAACTTAAATGAATTGACGG ACGGGCGGTGTGTAC
primer AAACTCAAAGGAATTGGCGG ACGGGCGGTGTGTGC
primer AAACTCAAAGGAATTGGCGG ACGGGCGGTGTGTAC
primer AAACTCAAAGGAATTGACGG ACGGGCGGTGTGTGC
primer AAACTCAAAGGAATTGACGG ACGGGCGGTGTGTAC
primer AAACTCAAATGAATTGGCGG ACGGGCGGTGTGTGC
primer AAACTCAAATGAATTGGCGG ACGGGCGGTGTGTAC
primer AAACTCAAATGAATTGACGG ACGGGCGGTGTGTGC
primer AAACTCAAATGAATTGACGG ACGGGCGGTGTGTAC
Is this correct or should I add “imaginary” barcodes? I tried to make group and the error is:
556091
Done.
Processing BFP2_S13_L001_R1_001.0ffastatemp (file 1 of 1) <<<<<
[ERROR]: make.contigs requires paired barcodes and primers. You can set one end to NONE if you are using an index file.
Making contigs…
[WARNING]: your sequence names contained ‘:’. I changed them to ‘_’ to avoid problems in your downstream analysis.
Thanks!!