Hi, all!
I met a problem about get.groups command, when I did the command I found that I didn’t have the group=MOCK.W7 file. What should I do then?
The command is like this: get.groups(fasta=W7.shhh.trim.unique.good.filter.unique.precluster.pick.pick.fasta, group=W7.shhh.good.pick.pick.groups, name=W7.shhh.trim.unique.good.filter.unique.precluster.pick.pick.names, group=MOCK.W7). I don’t have the MOCK.W7 file and don’t know how to generate it.
I’m waiting for your help! 
songer
9/02
Did you sequence a mock community with your samples? What was it’s group name?
Thanks for your reply. I did not know how to sequence a mock community with samples. Can I ignore this error analysis step? Is it necessary to the data process?
I’m waiting for the help. Thanks!
Yeah, to know the error rate for your data, you need to sequence a mock community in parallel. There is a growing chorus of papers suggesting that people include a mock community and report their error rates.
If you don’t do it, then you need to skip the analysis.
Pat