pcr.seqs pdiffs

Is is possible to set the number of pdiffs separately for the forward and reverse primers? I believe the current method is for the total number of pdiffs? so setting pdiffs = 3 means I’ll scrap a sequence with 2 mismatches to both the forward and reverse primer;

fpdiffs=2(match) rpdiffs=2(match)

but setting pdiffs = 6, means that I can get some pass that I do not want to

fpdiffs=1(match) rpdiffs=5(noMatch)

Any reasons as to why this is not good would be appreciated.


Thanks for the suggestion! We will add it to our list.