Current SILVA reference alignment files on Mothur site are from database version 102 which is over a year old by now (Feb 2010). Newest SILVA SSURef (ver 108) contains more than 100k extra sequences. Is there a plan to update these reference alignment files on this site or may I use the latest dataset from SILVA on our environmental samples although it contains both bacterial and eucaryotic references?
when will the new refernce file be avaiable? I am analyzing an Archaea data and there’s huge number of unclassied sequences, even the classifed ones are not well defined (only in the class level). I wonder weather its caused by the old silva reference or other reasons. Can a new reference be the solution? How can I use the downloaded SSURef_111_SILVA_04_08_12_opt in mothur? It’s really urgent for me. Thanks.
I would also be very interested in updated SILVA files for mothur. Would it be possible to tell us if there is an ETA for a new version of the files? Just so that I can decide if it makes sense to wait with this part of my analysis a bit and use the new files or go on using the old files and somehow deal with all the unclassified sequences. Thanks!