Mismatch when making Contigs

1

Hi everyone,

  • While running the make.contigs(file=stability.files) I received the error

Making contigs…
[WARNING]: name mismatch in forward and reverse fastq file. Ignoring, A00808_1070_H7LLYDRX2_2_2229_10248_3302.

just wanted to see if there was a way to filter this mismatch? I would appreciate any help or insight. Thank you in advance.

2

Hi Vane - is it possible that your sequence files were processed before you brought them into mothur? I’d strongly recommend going back to your sequence provider and getting the rawest possible fastq data off the sequencer that you can get.

Pat