Hey,
I am quite curious to know your thoughts on the Fadrosh paper. I know that Illumina also have a standard method of doing V3-4, although many sources suggest it would be better to stick with V4 or any other where you would have complete overlap of paired ends. What are your thoughts? Very tempted to sequence a larger fragment as many do and ignore the error scores? Or do the right thing, sacrifice resolution and do it right.
Kozich et al
Fadrosh et al