Dual-barcoding for thousands of samples

Feng_Guo · November 17, 2015, 2:21am

Hi,

I want to know whether there is a authorized list of dual barcoding indexes for thousands of samples. For example, 32*48 or even more.

pschloss · November 20, 2015, 12:41pm

We have a collection of primers that would get you up to 1536 total samples.

github.com

SchlossLab/MiSeq_WetLab_SOP/blob/master/MiSeq_WetLab_SOP_v4.md

# MiSeq Wet Lab SOP


**Detailed Title:** | 16S rRNA Sequencing with the Illumina MiSeq: Library Generation, QC, & Sequencing  
**Authors:**        | James Kozich, Patrick Schloss, Niel Baxter, Matt Jenior, & Charles Koumpouras  
**Original Date:**  | 25-March-2013  
**Version:**        | 5.0  
**Last Updated:**   | 2-December-2016  
**Updated By:**     | Charles Koumpouras  


------------------------------------
**1.0) Introduction and Workflow**
------------------------------------

***1.1) Introduction***

-   The Purpose of this protocol is to define the steps for 
    the preparation and sequencing of 16S rRNA gene sequence libraries 
    using the Illumina MiSeq sequencing platform, as described in the paper

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I'm not sure why you'd want to do more than that in a single run, but to each their own...

Feng_Guo · November 25, 2015, 1:25pm

Thanks!

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Dual-barcoding for thousands of samples

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