Hi there,
I currently have v.1.39.5 installed. I have a 16s dataset that was previously processed within mothur v.1.33.3, which I just ran again using the same pipeline, but with my current mothur version. One of the major differences I’ve noticed is that following trim.seqs for the same dataset, mothur v.1.33.3 obtained:
unique: 520,462
total: 2,854,782
whereas mothur version 1.39.5 obtained:
#unique: 851,096
total: 5,020,634
I’ve looked over the various updates that have occurred since v. 1.33.3, but I just can’t seem to pinpoint what change would result in nearly twice as many total sequences making it through the trimming stage. Any thoughts here? Thanks in advance!!