I split the distance file first. How to cluster now?

Feature requests
1

Hi

I had ~29GB distance file and I used cluster.split() to split it. Now I want to perform clustering before performing alpha, beta diversity calculations (following SOP 454).
Can someone tell me how to use these multiple split files as input to cluster program.

Thanks

2

Cluster.split will cluster the split files as long as cluster=t, which is the default. If you set cluster=f, you can also run the files one at a time with the cluster command, http://www.mothur.org/wiki/Cluster.

mothur > cluster(column=splitColumnFormattedDistanceFile1, names=splitNamesFile1)

3

Hi westcott

Thanks for the reply.
Here is what i have so far.
1- X.final.dist
2- X.final.names
3- X.final.dist.0.temp — X.final.dist.44.temp
4- X.final.names.0.temp — X.final.names.44.temp

Do you suggest me this
#cluster.split(column=X.final.dist.0.temp, name=X.final.names.0.temp,processors=1,large=T)
If yes then I don’t know how am I instructing cluster.split() to not run split step this time and only cluster?

4

Did mothur crash while you were running cluster.split the first time? Or did you set cluster=false?