How to change the cutoff for cluster

Commands in mothur
1

Now I am handling a 454 seqs data set. I need to set the cutoff value of the cluster command to 0.20. However, Mothur automatically change the cutoff value to 0.088 (For the dist.seqs command, I also set a cutoff value to 0.20).

How can I set the cutoff value to 0.20 for the cluster command?

Thanks.

2

I have got it after reading the manual again. Actually, the sequence distance is not far, so there is no meaning to cluster at a distance larger than 0.088.

3

http://www.mothur.org/wiki/Frequently_asked_questions#Why_does_the_cutoff_change_when_I_cluster_with_average_neighbor.3F

4

Thank you so much.