I am using the MiSeq SOP on a 18S data set. After QC (singletons out is the final step of it), I sub sampled my number of sequences to normalize using sub.sample(shared=myFile, size=50000)
I have 2 questions. First, what is the logic of the selection?
I would like to get the OTUs from this tailored shared file. But my trials might be failing cause I got a bug (that I plan to report).
I am working with the v.1.33.3
Thanks and have a nice day,