HELP! How to generate a '.group' file?

Commands in mothur
1

Hi Guys,
Do you know how to generate a ‘.group’ file?
I am so confused when I come to the screen.seqs command below:
mothur > screen.seqs(fasta=GQY1XT001.shhh.trim.unique.align, name=GQY1XT001.shhh.trim.unique.names, group=GQY1XT001.shhh.groups, end=27659, optimize=start, criteria=95, processors=2)
I have got a ‘.name’ file after the unique.seqs command, however, I cannot find a way to generate the group file!
Could you please give me a suggestion?
Thank you very very much!

2

If you’re doing working with 454/MiSeq data then the group file is generated by the trim.seqs() command during the SOP. If you’re not using this kind of data (if you’re using clone library data, or reads downloaded from GenBank, for instance) you’ll probably have your data split by groups across several files. In this case you can build a groups file for this using the make.group() command.

3

Dear Dwaite,
Thank you very very much! Handle it!