I would like to know whether comparisons of sequence abundance between samples were made after correcting for the unequal total number of sequences obtained from each sample.
In other words, if all abundance values within a sample are multiplied by the certain required factor so that the total normalized abundance for all samples becomes the same before the heatmap generation.

We normalize by relative abundance and then to the maximum relative abundance across groups. I’m learning a bit about how this is done for microarray data and suspect that we could probably do a better job with this.

Thank you.