Hi, I did pyrosequencing for my samples by Mi-Seq V4.
I made contig for the 10 samples.
After running the Dist.seqs, I got a big file which is around 40GB .list file.
I was running the Cluster, and the command screen looks like getting stuck.
Is there a problem for it? Is it normal by coming out with a 40GB file? If it is a problem, how can I fix it? and do I need to change some parameters? like cutoff value or what?
Thank you very much.
Your distance file is likely too large to cluster without a cutoff. You will want to run the cluster command with a cutoff.