I want to remove the sequences with only one read in each OTU and after that obtain the consensus sequeence of each Otu. I am using split.abund and get.oturep, however I need use a dist file and the dist file current is the dist without remove the singletons. What can I do them?
If you use method=abundance
in get.oturep
, then you don’t need a distance matrix.
Pat
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