function gene rdp and its taxonomy txt customised

Commands in mothur
1

Since we have a lot of way to assign 16s rDNA genes to their similar(97% to genus) sequence.
There are some common primers can amplify functional genes and these functional genes sequence sometimes can reflect their community level ,even to species degree. We can use the same way to sequence function gene, and blast them in NCBI, then find which genus they belong to.

I wonder if there any way that I can customised my reference database to a fasta and taxonomy txt by using Muther?

2

If you create a fasta and taxonomy file you can classify anything. You’ll just have to follow the model provided in the existing taxonomy files you should be good to go.

Pat