Fastq.info with only one read (Pacbio)

We’re using pacbio full-length 16S data in mothur to great success. However, when we do a multi-sample comparison, we have to construct all of the group files by hand. Fastq.info should be able to do this but it expects that the fastqFiles.file would have 3 columns: group, forward, and reverse read. With Pacbio there IS no forward/reverse read - the smartbell sequencing gives you a CSS (circular consensus). Is there a way that the next version of mothur could support single-read pacbio sequence in fastq.info (when the pacbio=T is set, of course)?

This topic was automatically closed 10 days after the last reply. New replies are no longer allowed.

Thanks for the suggestion. Yes, we can add this to our next version. You can follow the progress here, https://github.com/mothur/mothur/issues/649.