Error: Template is not aligned, aborting

Dear all,
I am using mothur v.1.43.0. I got an error (Template is not aligned, aborting.) in this command align.seqs(fasta=ecoli.v4.fasta, reference=silva.v132.align). could you advise me?

Hi there,

Are you sure that you are using the right file? Did you download silva.v132.align from the wiki or elsewhere? You might try downloading it again and try again.


Yeah I am sure. I tried many times, but it doesn’t work.

Could you post a screenshot of what you’re running and the error message you are getting?