Error: Template is not aligned, aborting

1

Dear all,
I am using mothur v.1.43.0. I got an error (Template is not aligned, aborting.) in this command align.seqs(fasta=ecoli.v4.fasta, reference=silva.v132.align). could you advise me?
Thanks

2

Hi there,

Are you sure that you are using the right file? Did you download silva.v132.align from the wiki or elsewhere? You might try downloading it again and try again.

pat

3

Hi,
Yeah I am sure. I tried many times, but it doesn’t work.

5

Could you post a screenshot of what you’re running and the error message you are getting?