Hi there!!! @pschloss @westcott
Can I take diffs=2 value for the pre.cluster step for read length 450 bp?
Thanks and regards,
DC7
Iād encourage you to read
Schloss PD, Gevers D, Westcott SL (2011). Reducing the effects of PCR amplification and sequencing artifacts on 16S rRNA-based studies. PLoS ONE. 6:e27310.
It gives a rationale for the number of diffs to use.
Pat
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