mothur

Diffs value for pre.cluster

Hi there!!! @pschloss @westcott
Can I take diffs=2 value for the pre.cluster step for read length 450 bp?

Thanks and regards,
DC7

Iā€™d encourage you to read

Schloss PD, Gevers D, Westcott SL (2011). Reducing the effects of PCR amplification and sequencing artifacts on 16S rRNA-based studies. PLoS ONE. 6:e27310.

It gives a rationale for the number of diffs to use.

Pat

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