demultiplexing

Commands in mothur
1

Hi Everyone,
I am wondering if mothur can do demultiplexing of raw data from Illumina.


Thanks!!!
2

Have you looked at make.contigs?

3

Thanks pschloss,
I have looked into this command but didn’t get much as the files which I have are original fastq file, barcode file and mapping file and it is single read.

Thanks!!!

4

then have you tried trim.seqs?

5

Thanks I got it!!!