demultiplexing

newbie · February 12, 2013, 2:29am

Hi Everyone,
I am wondering if mothur can do demultiplexing of raw data from Illumina.

Thanks!!!

pschloss · February 12, 2013, 3:43pm

Have you looked at make.contigs?

newbie · February 12, 2013, 4:05pm

Thanks pschloss,
I have looked into this command but didn’t get much as the files which I have are original fastq file, barcode file and mapping file and it is single read.

Thanks!!!

pschloss · February 12, 2013, 7:15pm

then have you tried trim.seqs?

newbie · February 14, 2013, 5:06pm

Thanks I got it!!!

Topic		Replies	Views
fastq, fasta and qual Commands in mothur	2	742	December 15, 2017
Shared miseq files (via basespace) already demultiplexed Commands in mothur	6	1385	July 11, 2017
MiSeq SOP using multiplexed R1.fastq and R2.fastq Commands in mothur	6	1021	October 9, 2017
make.contigs and 'clean' data Commands in mothur	1	627	February 27, 2018
splitting fastq files Commands in mothur	1	2696	November 8, 2013

demultiplexing

Related Topics