Hello all, I kinda have a lot of different 16S runs from chickens, swine, slaughter house, in vitro studies, etc.
I just want a way to put that all together and interrogate the database to be able to take the full use of all that spent money.
Here is what I have though of.
Independently, use Mothur on each experiment. The idea is when a new experiment is added to the database, Mothur do not need to be run from scratch. I would run Mothur just prior to making OTUs
Then, put all “cleaned” experiments together, using the merge command or add incoming new experiments to the “core file”.
run unique on the core file
make OTU from the core file
classify said OTU from the core file
Use the final classification and shared file for analysis purpose in a database from where I can pull out data according to certain metadata and run what I want to do from there: simply compare a new experiment with existing data, build up on a certain research topic or do machine learning for diagnostic purpose, etc.
Do you believe this is possible/useful?