Count file does not have group info in mothur

Commands in mothur
1

I’m struggling with an issue of my group names not being maintained. All I can think is that somehow my group names have an issue with the format? Example below:

_S010396.2 _S010396.2_R1.fastq _S010396.2_R2.fastq
_S010399.1 _S010399.1_R1.fastq _S010399.1_R2.fastq
_S010401.2 _S010401.2_R1.fastq _S010401.2_R2.fastq

When I get the the make.shared command, I get this error:
count file does not have group info in mothur

I have tried various solutions I have found on the forum, but nothing seems to have worked yet.

Thank You!
Danny

2

Hmmm, those file names are not ideal. When make.file does it’s thing it looks for the first _ and uses everything before it to name the group. So if your sequence files start with a _, I can imagine that would cause problems. Can you remove that initial underscore?

3

Yep, that was it! Thank you.

4

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