Considerations For Optimizing Microbiome Analysis Using a Marker Gene

Hi mothur friends,

we’ve just published a review that covers one critical aspect that has been overlooked by many researchers, namely, how to properly analyze 16S rRNA microbial data obtained through next-generation sequencing (NGS). We discuss the general pipeline used in all marker gene analyses, going from DNA extraction through getting clean, ready-to-analyze data, including issues concerning multitemplate PCR, the selection of hypervariable 16S rRNA regions and primers, the amplicon sequencing by NGS platforms, the removing of dubious and chimeric sequences, the clustering and classification of operational taxonomic units, and the correction by 16S copy number.

We are very interested in getting feedback and what better audience than the mothur community? If you have any questions or comments, please feel free to ask.

Best regards,