Hi
I am using mothur 1.36.0
When i am running the classify.otu command as given below i think all the sequences which remained unclassified in the clasify.seqs command are not being found in the tree file.
The following is the command
classify.otu(list=BP315.an.0.03.subsample.list, taxonomy=BP315.subsample.taxonomy, name=BP315.subsample.names, label=0.03, probs=f, basis=sequence, group=BP315.subsample.groups, reftaxonomy=gg_13_5_99.pds.tax)
The result
0.03 669
Warning: cannot find taxon g__HB2-32-21_unclassified in reference taxonomy tree at level 6 for SE.20101017.GY.D040S.BC.315.a.673605_83781. This may cause totals of daughter levels not to add up in summary file.
Warning: cannot find taxon g__HB2-32-21_unclassified in reference taxonomy tree at level 6 for SE.20101017.GY.D040S.BC.315.673602_99634. This may cause totals of daughter levels not to add up in summary file.
Warning: cannot find taxon g__HB2-32-21_unclassified in reference taxonomy tree at level 6 for SE.20101017.GY.D040S.BC.315.a.673605_168580. This may cause totals of daughter levels not to add up in summary file.
Warning: cannot find taxon g__HB2-32-21_unclassified in reference taxonomy tree at level 6 for SE.20101017.GY.D040S.BC.315.673602_280530. This may cause totals of daughter levels not to add up in summary file.
Warning: cannot find taxon g__HB2-32-21_unclassified in reference taxonomy tree at level 6 for SE.20101017.GY.D040S.BC.315.a.673605_306973. This may cause totals of daughter levels not to add up in summary file.
Warning: cannot find taxon g__HB2-32-21_unclassified in reference taxonomy tree at level 6 for SE.20101017.GY.D040S.BC.315.673602_313849. This may cause totals of daughter levels not to add up in summary file.
Warning: cannot find taxon g__HB2-32-21_unclassified in reference taxonomy tree at level 6 for SE.20101017.GY.D040S.BC.315.673602_373605. This may cause totals of daughter levels not to add up in summary file.
Warning: cannot find taxon g__HB2-32-21_unclassified in reference taxonomy tree at level 6 for SE.20101017.GY.D040S.BC.315.673602_389764. This may cause totals of daughter levels not to add up in summary file.
Warning: cannot find taxon g__HB2-32-21_unclassified in reference taxonomy tree at level 6 for SE.20101017.GY.D040S.BC.315.a.673605_390789. This may cause totals of daughter levels not to add up in summary file.
Warning: cannot find taxon g__HB2-32-21_unclassified in reference taxonomy tree at level 6 for SE.20101017.GY.D040S.BC.315.a.673605_467442. This may cause totals of daughter levels not to add up in summary file.
Warning: cannot find taxon g__HB2-32-21_unclassified in reference taxonomy tree at level 6 for SE.20101017.GY.D040S.BC.315.a.673605_491040. This may cause totals of daughter levels not to add up in summary file.
And it goes on and on.
I have never faced this issue before.
Can anyone explain what i am doing wrong here?
Thanks