mothur

Chimeravse error

I am receiving error when chimera.vsearch to remove chimeric sequences ,
I tried even dereplicate=t also , please help to sot it out
regards
SAbitha

chimera.vsearch(fasta=stability.trim.contigs.good.unique.good.filter.unique.precluster.fasta, count=stability.trim.contigs.good.unique.good.filter.unique.precluster.count_table, dereplicate=f)

Using 128 processors.
Unable to open /home/wia/vsearch. Trying input directory /home/wia/mothur/vsearch.
Unable to open /home/wia/mothur/vsearch. Trying default /home/wia/vsearch.
Unable to open /home/wia/vsearch. Trying mothur's executable location /home/wia/vsearch.
Unable to open /home/wia/vsearch. Trying mothur's tools location vsearch.
Checking sequences from /home/wia/mothur/stability.trim.contigs.good.unique.good.filter.unique.precluster.fasta ...

/******************************************/
Running command: split.groups(groups=SRR12264494-SRR12264495-SRR12264496-SRR12264497-SRR12264498-SRR12264499-SRR12264500-SRR12264501-SRR12264502-SRR12264503-SRR12264504-SRR12264506-SRR12264507-SRR12264508-SRR12264509-SRR12264510-SRR12264511-SRR12264512-SRR12264513-SRR12264514-SRR12264515-SRR12264518-SRR12264519-SRR12264520-SRR12264521-SRR12264523-SRR12264524-SRR12264525-SRR12264526-SRR12264527-SRR12264528-SRR12264529-SRR12264530-SRR12264531-SRR12264532-SRR12264533-SRR12264534-SRR12264535-SRR12264536-SRR12264537-SRR12264538-SRR12264539-SRR12264540-SRR12264541-SRR12264542-SRR12264543, fasta=/home/wia/mothur/stability.trim.contigs.good.unique.good.filter.unique.precluster.fasta, count=/home/wia/mothur/stability.trim.contigs.good.unique.good.filter.unique.precluster.count_table)

Using 128 processors.
Reducing processors to 46.
/******************************************/
Selecting sequences for group SRR12264495

/******************************************/
Selecting sequences for group SRR12264525

/******************************************/
Selecting sequences for group SRR12264524

/******************************************/
Selecting sequences for group SRR12264521

/******************************************/
Selecting sequences for group SRR12264504

/******************************************/
Selecting sequences for group SRR12264540

/******************************************/
Selecting sequences for group SRR12264528

/******************************************/
Selecting sequences for group SRR12264532

/******************************************/
Selecting sequences for group SRR12264527

/******************************************/
Selecting sequences for group SRR12264507

/******************************************/
Selecting sequences for group SRR12264523

/******************************************/
Selecting sequences for group SRR12264497

/******************************************/
Selecting sequences for group SRR12264530

/******************************************/
Selecting sequences for group SRR12264526

/******************************************/
Selecting sequences for group SRR12264514

/******************************************/
Selecting sequences for group SRR12264500

/******************************************/
Selecting sequences for group SRR12264543

/******************************************/
Selecting sequences for group SRR12264541

/******************************************/
Selecting sequences for group SRR12264501

/******************************************/
Selecting sequences for group SRR12264496

/******************************************/
Selecting sequences for group SRR12264539

/******************************************/
Selecting sequences for group SRR12264509

/******************************************/
Selecting sequences for group SRR12264542

/******************************************/
Selecting sequences for group SRR12264519

/******************************************/
Selecting sequences for group SRR12264510

/******************************************/
Selecting sequences for group SRR12264512

/******************************************/
Selecting sequences for group SRR12264520

/******************************************/
Selecting sequences for group SRR12264508

/******************************************/
Selecting sequences for group SRR12264511

/******************************************/
Selecting sequences for group SRR12264515

/******************************************/
Selecting sequences for group SRR12264502

/******************************************/
Selecting sequences for group SRR12264494

/******************************************/
Selecting sequences for group SRR12264535

/******************************************/
Selecting sequences for group SRR12264503

/******************************************/
Selecting sequences for group SRR12264499

/******************************************/
Selecting sequences for group SRR12264534

/******************************************/
Selecting sequences for group SRR12264529

/******************************************/
Selecting sequences for group SRR12264506

/******************************************/
Selecting sequences for group SRR12264537

/******************************************/
Selecting sequences for group SRR12264538

/******************************************/
Selecting sequences for group SRR12264533

/******************************************/
Selecting sequences for group SRR12264518

/******************************************/
Selecting sequences for group SRR12264531

/******************************************/
Selecting sequences for group SRR12264536

/******************************************/
Selecting sequences for group SRR12264498

/******************************************/
Selecting sequences for group SRR12264513

Selected 20 sequences from your fasta file.
/******************************************/

Selected 213 sequences from your fasta file.
/******************************************/

Selected 312 sequences from your fasta file.
/******************************************/

Selected 91 sequences from your fasta file.
/******************************************/

Selected 2984 sequences from your fasta file.
/******************************************/

Selected 1453 sequences from your fasta file.
/******************************************/

Selected 11 sequences from your fasta file.
/******************************************/

Selected 855 sequences from your fasta file.
/******************************************/

Selected 975 sequences from your fasta file.
/******************************************/

Selected 161 sequences from your fasta file.
/******************************************/

Selected 247 sequences from your fasta file.
/******************************************/

Selected 1275 sequences from your fasta file.
/******************************************/

Selected 1302 sequences from your fasta file.
/******************************************/

Selected 155 sequences from your fasta file.
/******************************************/

Selected 1378 sequences from your fasta file.
/******************************************/

Selected 685 sequences from your fasta file.
/******************************************/

Selected 27 sequences from your fasta file.
/******************************************/

Selected 109 sequences from your fasta file.
/******************************************/

Selected 1313 sequences from your fasta file.
/******************************************/

Selected 613 sequences from your fasta file.
/******************************************/

Selected 2022 sequences from your fasta file.
Selected 554 sequences from your fasta file.
/******************************************/

/******************************************/

Selected 1229 sequences from your fasta file.
/******************************************/

Selected 114 sequences from your fasta file.
/******************************************/

Selected 2968 sequences from your fasta file.
/******************************************/

Selected 4018 sequences from your fasta file.
/******************************************/

Selected 177 sequences from your fasta file.
/******************************************/

Selected 3354 sequences from your fasta file.
/******************************************/

Selected 445 sequences from your fasta file.
/******************************************/

Selected 1067 sequences from your fasta file.
/******************************************/

Selected 281 sequences from your fasta file.
/******************************************/

Selected 4360 sequences from your fasta file.
/******************************************/

Selected 438 sequences from your fasta file.
/******************************************/

Selected 752 sequences from your fasta file.
/******************************************/

Selected 1641 sequences from your fasta file.
/******************************************/

Selected 1760 sequences from your fasta file.
Selected 2005 sequences from your fasta file.
/******************************************/

/******************************************/

Selected 2175 sequences from your fasta file.
/******************************************/

Selected 1980 sequences from your fasta file.
/******************************************/

Selected 1640 sequences from your fasta file.
/******************************************/

Selected 217 sequences from your fasta file.
/******************************************/

Selected 3260 sequences from your fasta file.
/******************************************/

Selected 2902 sequences from your fasta file.
/******************************************/

Selected 252 sequences from your fasta file.
/******************************************/

Selected 649 sequences from your fasta file.
/******************************************/

Selected 2431 sequences from your fasta file.
/******************************************/


Output File Names: 
/home/wia/mothur/stability.trim.contigs.good.unique.good.filter.unique.precluster.SRR12264494.count_table
/home/wia/mothur/stability.trim.contigs.good.unique.good.filter.unique.precluster.SRR12264494.fasta
/home/wia/mothur/stability.trim.contigs.good.unique.good.filter.unique.precluster.SRR12264495.count_table
/home/wia/mothur/stability.trim.contigs.good.unique.good.filter.unique.precluster.SRR12264495.fasta
/home/wia/mothur/stability.trim.contigs.good.unique.good.filter.unique.precluster.SRR12264496.count_table
/home/wia/mothur/stability.trim.contigs.good.unique.good.filter.unique.precluster.SRR12264496.fasta
/home/wia/mothur/stability.trim.contigs.good.unique.good.filter.unique.precluster.SRR12264497.count_table
/home/wia/mothur/stability.trim.contigs.good.unique.good.filter.unique.precluster.SRR12264497.fasta
/home/wia/mothur/stability.trim.contigs.good.unique.good.filter.unique.precluster.SRR12264498.count_table
/home/wia/mothur/stability.trim.contigs.good.unique.good.filter.unique.precluster.SRR12264498.fasta
/home/wia/mothur/stability.trim.contigs.good.unique.good.filter.unique.precluster.SRR12264499.count_table
/home/wia/mothur/stability.trim.contigs.good.unique.good.filter.unique.precluster.SRR12264499.fasta
/home/wia/mothur/stability.trim.contigs.good.unique.good.filter.unique.precluster.SRR12264500.count_table
/home/wia/mothur/stability.trim.contigs.good.unique.good.filter.unique.precluster.SRR12264500.fasta
/home/wia/mothur/stability.trim.contigs.good.unique.good.filter.unique.precluster.SRR12264501.count_table
/home/wia/mothur/stability.trim.contigs.good.unique.good.filter.unique.precluster.SRR12264501.fasta
/home/wia/mothur/stability.trim.contigs.good.unique.good.filter.unique.precluster.SRR12264502.count_table
/home/wia/mothur/stability.trim.contigs.good.unique.good.filter.unique.precluster.SRR12264502.fasta
/home/wia/mothur/stability.trim.contigs.good.unique.good.filter.unique.precluster.SRR12264503.count_table
/home/wia/mothur/stability.trim.contigs.good.unique.good.filter.unique.precluster.SRR12264503.fasta
/home/wia/mothur/stability.trim.contigs.good.unique.good.filter.unique.precluster.SRR12264504.count_table
/home/wia/mothur/stability.trim.contigs.good.unique.good.filter.unique.precluster.SRR12264504.fasta
/home/wia/mothur/stability.trim.contigs.good.unique.good.filter.unique.precluster.SRR12264506.count_table
/home/wia/mothur/stability.trim.contigs.good.unique.good.filter.unique.precluster.SRR12264506.fasta
/home/wia/mothur/stability.trim.contigs.good.unique.good.filter.unique.precluster.SRR12264507.count_table
/home/wia/mothur/stability.trim.contigs.good.unique.good.filter.unique.precluster.SRR12264507.fasta
/home/wia/mothur/stability.trim.contigs.good.unique.good.filter.unique.precluster.SRR12264508.count_table
/home/wia/mothur/stability.trim.contigs.good.unique.good.filter.unique.precluster.SRR12264508.fasta
/home/wia/mothur/stability.trim.contigs.good.unique.good.filter.unique.precluster.SRR12264509.count_table
/home/wia/mothur/stability.trim.contigs.good.unique.good.filter.unique.precluster.SRR12264509.fasta
/home/wia/mothur/stability.trim.contigs.good.unique.good.filter.unique.precluster.SRR12264510.count_table
/home/wia/mothur/stability.trim.contigs.good.unique.good.filter.unique.precluster.SRR12264510.fasta
/home/wia/mothur/stability.trim.contigs.good.unique.good.filter.unique.precluster.SRR12264511.count_table
/home/wia/mothur/stability.trim.contigs.good.unique.good.filter.unique.precluster.SRR12264511.fasta
/home/wia/mothur/stability.trim.contigs.good.unique.good.filter.unique.precluster.SRR12264512.count_table
/home/wia/mothur/stability.trim.contigs.good.unique.good.filter.unique.precluster.SRR12264512.fasta
/home/wia/mothur/stability.trim.contigs.good.unique.good.filter.unique.precluster.SRR12264513.count_table
/home/wia/mothur/stability.trim.contigs.good.unique.good.filter.unique.precluster.SRR12264513.fasta
/home/wia/mothur/stability.trim.contigs.good.unique.good.filter.unique.precluster.SRR12264514.count_table
/home/wia/mothur/stability.trim.contigs.good.unique.good.filter.unique.precluster.SRR12264514.fasta
/home/wia/mothur/stability.trim.contigs.good.unique.good.filter.unique.precluster.SRR12264515.count_table
/home/wia/mothur/stability.trim.contigs.good.unique.good.filter.unique.precluster.SRR12264515.fasta
/home/wia/mothur/stability.trim.contigs.good.unique.good.filter.unique.precluster.SRR12264518.count_table
/home/wia/mothur/stability.trim.contigs.good.unique.good.filter.unique.precluster.SRR12264518.fasta
/home/wia/mothur/stability.trim.contigs.good.unique.good.filter.unique.precluster.SRR12264519.count_table
/home/wia/mothur/stability.trim.contigs.good.unique.good.filter.unique.precluster.SRR12264519.fasta
/home/wia/mothur/stability.trim.contigs.good.unique.good.filter.unique.precluster.SRR12264520.count_table
/home/wia/mothur/stability.trim.contigs.good.unique.good.filter.unique.precluster.SRR12264520.fasta
/home/wia/mothur/stability.trim.contigs.good.unique.good.filter.unique.precluster.SRR12264521.count_table
/home/wia/mothur/stability.trim.contigs.good.unique.good.filter.unique.precluster.SRR12264521.fasta
/home/wia/mothur/stability.trim.contigs.good.unique.good.filter.unique.precluster.SRR12264523.count_table
/home/wia/mothur/stability.trim.contigs.good.unique.good.filter.unique.precluster.SRR12264523.fasta
/home/wia/mothur/stability.trim.contigs.good.unique.good.filter.unique.precluster.SRR12264524.count_table
/home/wia/mothur/stability.trim.contigs.good.unique.good.filter.unique.precluster.SRR12264524.fasta
/home/wia/mothur/stability.trim.contigs.good.unique.good.filter.unique.precluster.SRR12264525.count_table
/home/wia/mothur/stability.trim.contigs.good.unique.good.filter.unique.precluster.SRR12264525.fasta
/home/wia/mothur/stability.trim.contigs.good.unique.good.filter.unique.precluster.SRR12264526.count_table
/home/wia/mothur/stability.trim.contigs.good.unique.good.filter.unique.precluster.SRR12264526.fasta
/home/wia/mothur/stability.trim.contigs.good.unique.good.filter.unique.precluster.SRR12264527.count_table
/home/wia/mothur/stability.trim.contigs.good.unique.good.filter.unique.precluster.SRR12264527.fasta
/home/wia/mothur/stability.trim.contigs.good.unique.good.filter.unique.precluster.SRR12264528.count_table
/home/wia/mothur/stability.trim.contigs.good.unique.good.filter.unique.precluster.SRR12264528.fasta
/home/wia/mothur/stability.trim.contigs.good.unique.good.filter.unique.precluster.SRR12264529.count_table
/home/wia/mothur/stability.trim.contigs.good.unique.good.filter.unique.precluster.SRR12264529.fasta
/home/wia/mothur/stability.trim.contigs.good.unique.good.filter.unique.precluster.SRR12264530.count_table
/home/wia/mothur/stability.trim.contigs.good.unique.good.filter.unique.precluster.SRR12264530.fasta
/home/wia/mothur/stability.trim.contigs.good.unique.good.filter.unique.precluster.SRR12264531.count_table
/home/wia/mothur/stability.trim.contigs.good.unique.good.filter.unique.precluster.SRR12264531.fasta
/home/wia/mothur/stability.trim.contigs.good.unique.good.filter.unique.precluster.SRR12264532.count_table
/home/wia/mothur/stability.trim.contigs.good.unique.good.filter.unique.precluster.SRR12264532.fasta
/home/wia/mothur/stability.trim.contigs.good.unique.good.filter.unique.precluster.SRR12264533.count_table
/home/wia/mothur/stability.trim.contigs.good.unique.good.filter.unique.precluster.SRR12264533.fasta
/home/wia/mothur/stability.trim.contigs.good.unique.good.filter.unique.precluster.SRR12264534.count_table
/home/wia/mothur/stability.trim.contigs.good.unique.good.filter.unique.precluster.SRR12264534.fasta
/home/wia/mothur/stability.trim.contigs.good.unique.good.filter.unique.precluster.SRR12264535.count_table
/home/wia/mothur/stability.trim.contigs.good.unique.good.filter.unique.precluster.SRR12264535.fasta
/home/wia/mothur/stability.trim.contigs.good.unique.good.filter.unique.precluster.SRR12264536.count_table
/home/wia/mothur/stability.trim.contigs.good.unique.good.filter.unique.precluster.SRR12264536.fasta
/home/wia/mothur/stability.trim.contigs.good.unique.good.filter.unique.precluster.SRR12264537.count_table
/home/wia/mothur/stability.trim.contigs.good.unique.good.filter.unique.precluster.SRR12264537.fasta
/home/wia/mothur/stability.trim.contigs.good.unique.good.filter.unique.precluster.SRR12264538.count_table
/home/wia/mothur/stability.trim.contigs.good.unique.good.filter.unique.precluster.SRR12264538.fasta
/home/wia/mothur/stability.trim.contigs.good.unique.good.filter.unique.precluster.SRR12264539.count_table
/home/wia/mothur/stability.trim.contigs.good.unique.good.filter.unique.precluster.SRR12264539.fasta
/home/wia/mothur/stability.trim.contigs.good.unique.good.filter.unique.precluster.SRR12264540.count_table
/home/wia/mothur/stability.trim.contigs.good.unique.good.filter.unique.precluster.SRR12264540.fasta
/home/wia/mothur/stability.trim.contigs.good.unique.good.filter.unique.precluster.SRR12264541.count_table
/home/wia/mothur/stability.trim.contigs.good.unique.good.filter.unique.precluster.SRR12264541.fasta
/home/wia/mothur/stability.trim.contigs.good.unique.good.filter.unique.precluster.SRR12264542.count_table
/home/wia/mothur/stability.trim.contigs.good.unique.good.filter.unique.precluster.SRR12264542.fasta
/home/wia/mothur/stability.trim.contigs.good.unique.good.filter.unique.precluster.SRR12264543.count_table
/home/wia/mothur/stability.trim.contigs.good.unique.good.filter.unique.precluster.SRR12264543.fasta

/******************************************/
Reducing processors to 46.
sh: vsearch: command not found
sh: vsearch: command not found
Error in reading your fastafile, at position -1. Blank name.
Error in reading your fastafile, at position -1. Blank name.
sh: vsearch: command not found
sh: vsearch: command not found
It took 1 secs to check 0 sequences.

Hi,

It looks like you don’t have vsearch installed. When you downloaded mothur and copied things around, is it possible that you deleted the file?

Pat

No Sir
It is there mothur folder as executable file

thanks and regards

Can you post a screenshot of running ls from the command prompt where you are running mothur from?