chimera.slayer template for fungi

Feature requests
1

hello all,
i am analyzing 454-pyrosequencing results for fungal ITS and SSU subunit and i am looking for a template to put in the chimera.slayer.
if you can please tell me where is can find it, or how i can create a chimera slayer template for the fungi based on my SSU and ITS data base that i have.

thank you in advance,
Ashraf

2

Unfortunately, there isn’t one. You’d have to recreate it yourself using the outline described by Hass and colleagues in the ChimeraSlayer paper. Alternatively, if you have abundance information for each sequence, you could include the name file with your fasta file and use reference=self to use your more abundant sequences as the references.