When I try to run the Chimera Slayer, the program is saying that the sample sequence length is not the same length as the template sequences.
How can I go about making sure those are the same sequence length?
Here’s the summary.seqs for some of the files I have, and I am using the Silva.gold.align as the template for the Chimera Slayer.
mothur > summary.seqs(fasta=baileygood.unique.good.filter.unique.fasta) Start End NBases Ambigs Polymer Minimum: 1 1191 422 0 4 2.5%-tile: 1 1191 444 0 4 25%-tile: 1 1191 484 0 5 Median: 1 1191 489 0 5 75%-tile: 1 1191 500 0 5 97.5%-tile: 1 1197 510 0 6 Maximum: 1 1321 586 0 7 # of Seqs: 11464
Output File Name:
baileygood.unique.good.filter.unique.fasta.summary
mothur > summary.seqs(fasta=baileygood.unique.good.filter.fasta)
Start End NBases Ambigs Polymer
Minimum: 1 1191 422 0 4
2.5%-tile: 1 1191 444 0 4
25%-tile: 1 1191 484 0 5
Median: 1 1191 489 0 5
75%-tile: 1 1191 500 0 5
97.5%-tile: 1 1197 510 0 6
Maximum: 1 1321 586 0 7
of Seqs: 11517
Output File Name:
baileygood.unique.good.filter.fasta.summary
mothur > summary.seqs(fasta=baileygood.unique.good.align)
Start End NBases Ambigs Polymer
Minimum: 1044 13862 422 0 4
2.5%-tile: 1044 13862 444 0 4
25%-tile: 1044 13862 484 0 5
Median: 1044 13862 489 0 5
75%-tile: 1044 13862 500 0 5
97.5%-tile: 1044 13875 510 0 6
Maximum: 1044 21276 586 0 7
of Seqs: 11517
Output File Name:
baileygood.unique.good.align.summary