biom file input in Mothur

Hi,
i am new to Mothur, so i dont have enough knowledge about it. I want to analyze biom file (biom format) which i took from QIIME.
Is Mothur accepts QIIME biom file ? If it does, then how can i import in Mothur for Alpha and Beta diversity.

Best !
Shashank

You can use make.shared to generate a shared file from a biom file

http://www.mothur.org/wiki/Make.shared

Thank you for the information.

when i used the command in Mothur, i got an error
[b]mothur > make.shared(biom=F16_closed_ref.biom)


Output File Names: F16_closed_ref.shared
mothur >

[ERROR]: You are missing (
Invalid.[/b]

and when i saw the file, its empty. How can i resolve this issue ?

Best!
shashank

The error message is because you added an extra line ending when you copied and pasted or when you were typing. It’s not meaningful. You’re saying that F16_closed_ref.shared is empty? What version number of mothur and operating system are you using?

Pat

i tried again by writing the above command, now i won’t get any error, though the output file i obtained is F16_closed_ref.shared, which is empty.
the version i am using is
mothur v.1.25.0

Distributor ID: Ubuntu
Description: Ubuntu 12.04.5 LTS
Release: 12.04
Codename: precise

You should upgrade your version of mothur, http://www.mothur.org/wiki/Download_mothur.