Hi,
Sorry for too many post.
Since I checked tutorial again. I found something wired.
On analysis steps, based on tutorial…
mothur > count.groups(shared=final.opti_mcc.shared)
mothur > sub.sample(shared=final.opti_mcc.shared, size=2403)
mothur > rarefaction.single(shared=final.opti_mcc.shared, calc=sobs, freq=100)
However, when I ran sub.sample commands, it gave me the file named “final.opti_mcc.0.03.subsample.shared”
Should rarefaction.single commans like below?
mothur > rarefaction.single(shared=final.opti_mcc.0.03.subsample.shared, calc=sobs, freq=100)
Best,
Jun