I have a problem, I do not know how to start the analysis of my data, because they are all in a single file fasta (R1.fasta and R2.fasta), I have doubts about how I know to differ the groups, because in them they have 5 different groups. How do I start the analysis (make.contigs?) And then how will I know who is who.
Thanks so much.
I’d encourage you to ask your sequence provider for the raw fastq data. They are likely making sequence curation decisions to you that are not ideal. Life will be easier for you if you can start from the beginning.
Ok, thank you.
I’ll go look for more information.
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