I found this following [WARNING] message after an “align.seqs” step. Can anyone please tell me should I specially deal with it? Or, should I keep as it is?
It took 193 secs to align 144966 sequences. [WARNING]: 49 of your sequences generated alignments that eliminated too many bases, a list is provided in /home/dc7/Desktop/mothur_analysis/prjna_612042_obese_fecal/merge.paired.trim.contigs.good.unique.flip.accnos. [NOTE]: 25 of your sequences were reversed to produce a better alignment. It took 193 seconds to align 144966 sequences.
Thanks and regards,