I am new to mothur, but I have been working with sequencing files for quite some time now.
I have ~450 ABI files (.ab1 format) of 16s gene sequences. I got the files by sending 16s rRNA of single bacterial colonies for sequencing. I wish to analyse them using mothur, so I have two questions regarding that:
- Can I import these files and also use the quality data embedded in it so mothur could analyze it ? what I mean is that I want to asses the quality of the files using mothur. I know how to do that with sff and qual files but how do I do it if I only have one file (.ab1) that contains both sequence and quality ?
- I have 450 files to process. I know I that for loading and merging I need to write each and every file name to load, and that I can use the “-” sign in between file names, but what if I have so many files ? is there a way to tell mother load “*.abi” or merge it ?
Thank you all so much, this software do look awesome and I hope I can use it to my needs!