Hi mothur users
I have an issue I would like to know your thoughts on. I finished the fungal analysis following mothur SOP. The sequences are from the ITS2 region and I used the UNITE database as a reference database.
The problem is that one of the large size OTUs has come as k_unclassified fungi. I’ve got the representative sequence for this particular otu but it has no matches or similarities in UNITE and other databases.
I’m mainly looking for yeasts, and not sure if UNITE database has a good coverage for yeasts?. I know it covers the entire ITS2 region.
What do you think the problem might be?
Appreciate your help