Hi hi, another question on the trim.seqs command… I am analyzing MiSeq data and following the MiSeq SOP… I would like to do a quality control on my sequences however no .qual file is produced… How would one screen for sequences with a minimum quality score?
Thank you!
There is no way to do this presently. The problem is that there is no obvious method for deriving a consensus quality score. If you read the paper and go through the SOP as we describe, there really should be no need for consensus quality scores.
Pat
Hi Dr. Schloss,
Ah I see! Well thank you that clears things up!
Cheers!