Yes, I used Caporasso primer sets. It is great to know that I do not need to redo my entire analysis if trimming is not needed in my case.
I was told by sequencing center that my raw data was not trimmed off the sequencing adapters. If I do not need to trim barcode, pad, link, and primer, I should not need to trim the sequencing adapters, right? since adapters (e.g., forward adapter) are located way upstream of the primer sequence, correct?
Thank you very much. I would appreciate your confirmation.
Then the sequencing center must have used custom sequencing primers because the Caporasso primers do not contain any Illumina priming sites. Therefore the target primers were not sequenced.