Hi, I have been following the following SOP workflow to work with 16s RNA gene sequences. According to the approach of my project I would like to find differences between groups and therefore I plan to use ANOVA.
My question is if I already subsampled the sequences in the mcc.shared file do I have to do it again (in terms of the relative abundance data, not sequences) to avoid sequencing depth errors or is the subsampling already implicit?
It is possible to get a single subsampled OTU table?
Thanks in advance